Monkeypox Virus B6R Protein (2022 Strain, Myc and His tags)
Catalog Number:
F64X96
Description:
Monkeypox virus (MPXV) is a member of the Orthopoxvirus of the family Poxviridae. B6R extracellular enveloped virus (EEV) membrane protective antigen (Cop-B5R) complement interacts with multiple proteins located both on the membranes of infected cells and on EEV envelope for multiple functions (such as intracellular wrapping), similar to Vaccinia virus strain Copenhagen B5R.
A recombinant form of monkeypox virus protein B6R (Genbank: ON563414.3, MPXV_USA_2022_MA001; ON838178.1, isolate SI2022_S7) was produced by insect cells, followed by purification. This product contains ectodomain only (19-275), N-terminal Myc tag and C-terminal 6 hexa-histidine tag with amino acid sequence available upon request. Amino acid sequence for B6R: Tctvptmnnakltstetsfndkqkvtftcdsgyhsldpnavcetdkwkyenpckkmctvsdyvselydkplyevnstmtlscngetkyfrceekngntswndtvtcpnaecqplqlehgscqpvkekysfgeymtincdvgyevigvsyisctanswnvipscqqkcdipslsnglisgstfsiggvihlscksgftltgspsstcidgkwnpilptcvrsneefdpvddgpddetdlsklskdvvqyeqeiesle. This product is predicted to be a molecular mass of ~32 kDa.
This product has been run on SDS-PAGE with reduced condition. We also evaluated its binding to antibodies of anti-His or anti-Myc.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others
Source:
Insect cells
Biosafety Level:
BSL-1
Organism:
Monkeypox virus 2022 Strain
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
