SARS-CoV (2003 Strain) Spike Receptor Binding Domain (RBD) (His and HA tags)
Catalog Number:
V96K50
Description:
A recombinant form of the receptor binding domain (RBD with amino acids 334 to 516) of the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus (SARS-CoV), (GenBank: AY278554.2) was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains C-terminal 8 hexa-histidine tag and HA tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition. We also evaluated the binding to SARS specific mAb (clone RV38, Cat. No. X42K12) and published 80R and CR3022 antibodies in ELISAs, see results.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
Source:
HEK293
Biosafety Level:
BSL-1
Organism:
SARS-Related Coronavirus (virus caused 2003 outbreak)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.















