SARS-CoV (2003 strain) Trimeric Spike protein (His and Twin-Strep tags)
Catalog Number:
Z18G29
Description:
A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov (GenBank: AY278554.2) was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein, and also had a pair of mutations (K968P and V969P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition and Native blue gels, with results proving trimerization and glycosylation. We also evaluated the binding of one SARS Positive control mAb (clone RV38, Cat. No. X42K12), published 80R and CR3022 antibodies, as well as ACE2-Fc in ELISAs, see results.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
Source:
HEK293
Biosafety Level:
BSL-1
Organism:
SARS-Related Coronavirus (virus caused 2003 outbreak)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
