Human Anti-Papillomavirus L1 Antibody IgG Titer Serologic Assay Kit (HPV16 & 18)
Catalog Number:
C96J69
Background:
Human papillomavirus (HPV) is a member of the papoviridae virus. HPV infection is the primary cause of cervical cancer. More than 40 subtypes have been identified, with the high-risk subtypes such as HPV types 16, 18, 31, 33, 34, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, and 70. HPV16 is the cause of ~54% of invasive cervical cancers and HPV18 is known for the 17% of worldwide HPV infection. The subtypes of HPV are defined by the sequence variation on L1 protein, the major element of the viral capsid and component of approved vaccines. A rapid and effective assay kit detecting the levels of anti-HPV16 and HPV18 in human serum can facilitate research on antibodies produced in response to HPV infection or evaluate the vaccine-elicited antibody response.
Applications:
This kit is developed for serologic test for human IgG titer of Anti-HPV L1 antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Description:
This kit was tested to bind to known monoclonal antibody samples starting from concentration of 10 μg/ml and serum samples starting from 100-fold dilution.
Ordering:
C96J69-16 for plate pre-coated with L1 of HPV16 strain
C96J69-18 for plate pre-coated with L1 of HPV18 strain
Biosafety Level:
BSL-1
Organism:
Human papillomavirus (HPV)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-HPV L1 human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with L1 protein, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml/well)
- Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
