Human Anti-Influenza Hemagglutinin (HA) Antibody IgG Titer Serologic Assay Kit
Catalog Number:
Z48C46
Background:
Influenza viruses, RNA viruses of the family Orthomyxoviridae, are disease-causing agents in humans and animals. Influenza A viruses are classified using the antigenic characteristic of the envelope glycoproteins haemagglutinin (HA) and neuraminidase (NA). Influenza A virus subtype H3 (such as H3N1 or H3N2) cause seasonal epidemics in the human population. Influenza A virus subtype H5 (such as H5N1) usually causes the disease avian influenza (often referred to as “bird flu”), but occasionally infect humans. Compared with Influenza A, Influenza B viruses attribute lower morbidity and mortality rates, and represented by Victoria lineage.
Applications:
This kit is developed for serologic test for human IgG titer of Anti-Flu hemagglutinin (HA)-specific antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Description:
These kits were tested to bind to published antibody, 100F4 (H5-specific, Nat Commun. 2015:6:8855), clone RV28 (H5-specific, Cat. No. E3V21) or clone RV14 (Pan-Flu A and B, Cat. No. F91A45), see figures.
Ordering:
Z48C46-A-H3 for plate pre-coated with HA of Influenza A H3 lineage from A/H3N2 (A/Darwin/9/2021)
Z48C46-A-H5 for plate pre-coated with HA of Influenza A H5 lineage from A/Pelecanus/Peru/VFAR-140/2022(H5N1)
Z48C46-B-V for plate pre-coated with HA of Influenza B Victoria lineage from B/District Of Columbia/17/2019
Biosafety Level:
BSL-1
Organism:
Influenza (flu)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Flu HA human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with flu HA, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
- Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
Recommendations:
Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Anti-Influenza HA Neutralizing Monoclonal Antibody (Pan-Flu A and B) clone RV14 (Cat. No. F91A45).
