Human Anti-Varicella-Zoster virus (VZV) gE Protein Antibody IgG Titer Serologic Assay Kit

Human Anti-Varicella-Zoster virus (VZV) gE Protein Antibody IgG Titer Serologic Assay Kit

Catalog Number:

B93H1

Background:

The human Anti-Varicella-Zoster virus (VZV) is associated with two distinct diseases: childhood chickenpox (varicella) and shingles (zoster). After a primary infection, VZV remains latent in the sensory ganglia throughout the person’s lifetime and may reactivate to cause shingles. VZV DNA encodes seven glycoproteins (gB, gC, gE, gH, gI, gK, and gL) and gE is a dominant antigen of an approved vaccine (RZV, Shingrix, GSK). A rapid and effective assay kit detecting the levels of anti-VZV gE in human serum can facilitate research on antibodies produced in response to VZV infection or vaccine (approved or candidate).

Applications:

This kit is developed for serologic test for human IgG titer of Anti-VZV gE antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.

Description:

These kits were tested to bind to monoclonal antibodies or human sera, see figures.

Biosafety Level:

BSL-1

Organism:

Human Varicella-Zoster virus (VZV)

Assay Principles:

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Varicella-Zoster virus (VZV) gE human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with gE protein, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml/well)
2. Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml/well). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml/well) and incubate at room temperature for 10-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.

Recommendations:

Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Anti-Varicella-Zoster virus (VZV) gE Antibody (clone RV37, Cat. No. J65C52).