Mouse Anti-Cytomegalovirus gB Protein Antibody IgG Titer Serologic Assay Kit

Mouse Anti-Cytomegalovirus gB Protein Antibody IgG Titer Serologic Assay Kit

Catalog Number:

X66D33

Background:

Human cytomegalovirus (HCMV) is a pathogen in immunocompromised hosts and also the most common viral infection acquired in utero. The virus can cause severe disease in transplant recipients and infants, leading hearing loss or central nervous system damage. HCMV gB is an essential viral envelope glycoprotein which is involved in the early events of infection, viral entry and cell-to-cell spread. A rapid and effective assay kit detecting the levels of anti-HCMV gB (trimeric form) in mouse serum can facilitate research on antibodies produced in response to HCMV infection or vaccine candidates.

Applications:

This kit is developed for serologic test for mouse IgG titer of Anti-HCMV gB antibody in serum/plasma or purified mouse antibody (monoclonal & polyclonal) in vitro. It is for research use only.

Biosafety Level:

BSL-1

Organism:

Human cytomegalovirus (HCMV)

Assay Principles:

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-HCMV (Towne strain) mouse IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with trimeric gB glycoprotein, Positive control, Negative Control, an HRP-Anti-Mouse IgG secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml/well)
2. Dilute the Secondary antibody HRP-Anti-Mouse IgG by wash solution and add to each well (0.1 ml/well). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml/well) and incubate at room temperature for 10-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.