Mouse Anti-HIV-1 Antibody IgG Titer Serologic Assay Kit (Trimeric GP140)
Catalog Number:
H52F37
Background:
HIV (mouse immunodeficiency virus) is a virus that attacks the body’s immune system. HIV-1 lineage is more prevalent than HIV-2. A rapid and effective assay kit detecting the levels of anti-HIV-1 glycoprotein (GP) in mouse serum can facilitate research on antibodies produced in response to HIV-1 virus infection or vaccines.
Applications:
This kit is developed for serologic test for mouse IgG (including IgG1, IgG2 IgG3, IgG4) titer of Anti-HIV-1 GP antibody in serum/plasma or purified mouse antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Ordering:
H52F37-1 for plate pre-coated with trimeric GP140 of 16055 strain
H52F37-2 for plate pre-coated with trimeric GP140 of BG505 strain
H52F37-3 for plate pre-coated with trimeric GP140 of JRFL strain
H52F37-4 for plate pre-coated with trimeric GP140 of YU2 strain
Biosafety Level:
BSL-1
Organism:
HIV-1 virus
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-HIV-1 GP mouse IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with trimeric GP140 protein, Positive control, Negative Control, an HRP-Anti-Mouse IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
- Dilute the Secondary antibody HRP-Anti-Mouse IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
