Mouse Anti-Herpes Simplex Virus (HSV) gD Protein Antibody IgG Titer Serologic Assay Kit (Subtypes 1&2)
Catalog Number:
Y45N73
Background:
Herpes simplex virus (HSV) is ubiquitous human pathogen. Two main subtypes, HSV-1 and HSV-2, typically causes oral cold sores and genital lesions, respectively. Glycoprotein D (gD) of HSV binds to a host cell surface receptor as the prominent vaccine target. A rapid and effective assay kit detecting the levels of anti-HSV in mouse serum can facilitate research on antibodies produced in response to vaccine candidates.
Applications:
This kit is developed for serologic test for mouse IgG titer of Anti-HSV gD antibody in serum/plasma or purified mouse antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Ordering:
Y45N73-1 for plate pre-coated with gD of HSV-1 strain
Y45N73-2 for plate pre-coated with gD of HSV-2 strain
Biosafety Level:
BSL-1
Organism:
Herpes simplex virus (HSV)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-HSV gD mouse IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with gD glycoprotein, Positive control, Negative Control, an HRP-Anti-Mouse IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml/well)
- Dilute the Secondary antibody HRP-Anti-Mouse IgG by wash solution and add to each well (0.1 ml/well). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml/well) and incubate at room temperature for 10-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
