Human CTLA-4 protein (C122S mutation), Myc and His tags

Catalog Number:

A96M36

Description:

Human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a member of the CD28-B7 superfamily and acts as a co-inhibitory receptor for T-cell activation. A recombinant form (Ala 37 – Phe 162) of CTLA-4 protein (Accession: P16410) fused with Myc tag followed by a 6-His tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The mutation of C122S is to generate the native dimeric conformation. The purity has been examined >95% as determined by reducing SDS-PAGE, where protein with a calculated MW of ~28 kDa. We evaluated its binding to a CTLA-4-specific antibody (lpilimumab similar) clone RV96 (Cat.No. N7D25), two tag-specific antibodies (Cat.No. R44J3 for His tag; Cat.No. M52T82 for Myc tag), CD80 and CD86 in ELISAs, see results.

Protein Synonyms:

CTLA4, CD152

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Ala 37 – Phe 162 with C122S mutation

Tag:

Myc and His tags

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.