Human alpha-Klotho (KLA) protein, Myc and His tags

Catalog Number:

D44P67

Description:

Human alpha-Klotho (α-Klotho) is predominantly expressed in the kidney, brain and exerts anti-aging effects. A recombinant form (Glu 34 – Ser 981) of α-Klotho protein (Accession: Q9UEF7.2) fused with Myc tag followed by a 6-His tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined >90% as determined by reducing SDS-PAGE, where protein with a calculated MW of ~118 kDa. We evaluated its binding to a His tag-specific antibody (Cat.No. R44J3) and Myc tag-specific antibody Cat.No. M52T82) in ELISAs, see results.

Protein Synonyms:

alpha Klotho, alpha-klotho, AKL, KLA, klotho alpha like, klotho alpha-like protein

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Glu 34 – Ser 981

Tag:

Myc and His tags

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.