Quick Human HER3 /ErbB3 ELISA Kit

Catalog Number:

B64U94

Background:

Human epidermal growth factor receptor 3 (HER3 /ErbB3) is an important biomarker and tumor marker used for targeted treatments of specific types of cancer.

Applications:

This kit is developed to quickly titrate human HER3 /ErbB3 sample in high throughput. It is for research use only.

Description:

These kits were validated using human HER3 /ErbB3 protein (see figure). The detection linear range is 2.3 ng/ml – 10 µg/ml; Sensitivity is 2.3 ng/ml.

Biosafety Level:

BSL-1

Organism:

Human epidermal growth factor receptor 3 (HER3 /ErbB3)

Assay Principles (Take neutralizing antibody as example):

This kit is developed for a sandwich ELISA format to specifically capture human HER3 /ErbB3 protein in serum, plasma, tissue homogenates, cell culture supernatant and other biological fluids. The whole process should take about 2.5 hours. An antibody specific for HER3 /ErbB3 has been pre-coated onto a detachable 96-well microtiter plate. The standards and test samples are added into the wells and the HER3 /ErbB3 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with HRP labeled detector antibody, which binds the captured HER3 /ErbB3 present in each well. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-colored product that changes to yellow after addition of stop solution. The density of yellow is proportional to the amount of HER3 /ErbB3 captured in each well. The concentration of HER3 /ErbB3 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

The kit consists of detachable 96-well plate pre-coated with HER3 /ErbB3 -specific antibody, HER3 /ErbB3 protein standard, HRP labeled detector antibody, TMB and sample dilution/wash/stop buffer.

Brief protocol (detailed protocol is provided with the product):

1. Add your samples containing human HER3 /ErbB3 and provided purified HER3 /ErbB3 protein standard into pre-coated plate (or strips based on sample size). Incubate at 37°C for 1 hour.
2. Wash wells. Add HRP labeled detector antibody to wells. Incubate at room temperature for 1 hour.
3. Wash wells. Add TMB and stop solution. Read absorbance (OD) at 450 nm. The OD Value reflects the amount of HER3 /ErbB3 in the samples, which is then quantified against HER3 /ErbB3 standard curve to calculate titer.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.