Human CD80 Protein, Fc tag

Catalog Number:

B35S71

Description:

Human CD80 (also known as B7-1) on the surface of antigen-presenting cells (APCs) binds to CTLA-4 (Cytotoxic T-Lymphocyte Associated Protein 4) on T cells. A recombinant form (Val 35 – Asn 242) of CD80 protein (GenBank: NP_005182.1) fused with human IgG1 Fc (Fc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined as determined by reducing SDS-PAGE, where protein with a calculated MW of ~ 55 kDa (including tags) migrates as 65-90 kDa. We evaluated its binding to CTLA-4 protein (Cat.No. A96M36) in ELISAs, see results.

Protein Synonyms:

CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Val 35 – Asn 242

Tag:

human IgG1 Fc

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.