Influenza B Nucleoprotein (NP) protein (Yamagata lineage)

Catalog Number:

X1M24

Description:

Influenza (Flu) Nucleocapsid protein or nucleoprotein (NP) is the most abundant viral protein in infected cells, therefore the NP has been used for anti‑flu drug development or flu diagnosis. A recombinant Influenza B NP from Yamagata lineage (B/Minnesota/15/2020) was produced by insect cells, followed by purification. This product contains N-terminal Myc tag and C-terminal human Fc tag followed by a 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition, where the target protein with a calculated MW of ~90 kDa (including tags) was shown. We evaluated its binding to His tag-specific antibody (Cat.No. J96C86) and Myc tag-specific antibody (Cat.No. M52T82) in ELISAs, see results. Human Polyclonal IgG (Cat.No. P61W44) showed the stronger binding to Flu A NP (H3N2 strain) (Cat.No. G17H33) than this protein (data not shown).

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others

Source:

Insect cells

Biosafety Level:

BSL-1

Organism:

Influenza B

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.