Human Carcinoembryonic Antigen (CEA) Protein, Fc Tag or Tag free

Catalog Number:

A28K97

Description:

Human Carcinoembryonic Antigen (CEA) is a sensitive tumor biomarker for gastrointestinal cancer, and it is widely expressed in colorectal cancer (CRC) tissue and serum. Human carcinoembryonic antigen-related cell adhesion molecule 5 (CEAM5) with AA Lys 35 – Ala 685 (Accession: AAA51967.1 or P06731) fused with a HRV3C cleavage site and human IgG1 Fc (Fc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. Sequence is available upon the request. The purity has been examined >95% as determined by reducing SDS-PAGE, where protein migrates as 102-120 kDa. We evaluated its binding to a CEA-specific antibody clone RV64 (Cat.No. Y62E54) in ELISAs, see results. The protein can be cleaved by HRV3C protease for tag free.

Protein Synonyms:

Human Carcinoembryonic antigen-related cell adhesion molecule 5 or CD66e, CEAM5, Carcinoembryonic antigen, CEACAM5, CD66e, Meconium antigen 100, Carcinoembryonic antigen-related cell adhesion molecule 5, CEA

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Lys 35 – Ala 685

Tag:

human IgG1 Fc tag

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.