Human Anti-Monkeypox Antibody IgG Titer Serologic Assay Kit (Envelope GP)

Human Anti-Monkeypox Antibody IgG Titer Serologic Assay Kit (Envelope GP)

Catalog Number:

A5G21

Background:

One of symptoms of Monkeypox virus infection is rash. A rapid and effective assay kit detecting the levels of anti-Monkeypox envelope glycoprotein (GP) in human serum can facilitate research on antibodies produced in response to Monkeypox virus infection or vaccines.

Applications:

This kit is developed for serologic test for human IgG titer of Anti-Monkeypox envelope GP antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.

Description:

This kit was tested to bind to the included positive and negative antibodies starting from concentration of 10 μg/ml.

Ordering:

A5G21-A for plate pre-coated with Monkeypox envelope GP A35R of 2022 strain (Genbank: ON563414.3, MPXV_USA_2022_MA001)

A5G21-B for plate pre-coated with Monkeypox envelope GP B6R of 2022 strain (Genbank: ON563414.3, MPXV_USA_2022_MA001)

Biosafety Level:

BSL-1

Organism:

Monkeypox virus

Assay Principles:

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Monkeypox envelope GP human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with envelope GP protein, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
2. Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.

Recommendations:

Customers who intend to use this kit (A5G21-A) to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Anti-Monkeypox A33 Monoclonal Antibody (Cat. No. Q94A10).