Mouse Anti-Ebola Antibody IgG Titer Serologic Assay Kit (Trimeric GP)

Mouse Anti-Ebola Antibody IgG Titer Serologic Assay Kit (Trimeric GP)

Catalog Number:

P47J20

Background:

Ebola virus caused several outbreaks with the most widespread one at 2013-2016. There are several strains identified, including Sudan (SUDV), Zaire (EBOV) and others. A rapid and effective assay kit detecting the levels of anti-Ebola glycoprotein (GP) in mouse serum can facilitate research on antibodies produced in response to Ebola infection or vaccines.

Applications:

This kit is developed for serologic test for mouse IgG (including IgG1, IgG2 IgG3, IgG4) titer of Anti-Ebola GP antibody in serum/plasma or purified mouse antibody (monoclonal & polyclonal) in vitro. It is for research use only.

Ordering:

P47J20-S for plate pre-coated with trimeric GP of Ebola Sudan strain

P47J20-Z for plate pre-coated with trimeric GP of Ebola Zaire Makona strain

Biosafety Level:

BSL-1

Organism:

Ebola Sudan or Zaire strain

Assay Principles:

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Ebola GP mouse IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with trimeric GP protein, Positive control, Negative Control, an HRP-Anti-Mouse IgG secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
2. Dilute the Secondary antibody HRP-Anti-Mouse IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.