SARS-CoV-2 Beta (B.1.351 variant) Trimeric Spike (His and Twin-Strep tags)
Catalog Number:
R12T26
Description:
A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (GenBank: MN908947) bearing mutations of L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V, identified in B.1.351 (Beta variant), was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition with result showing pure and expected size. We also evaluated the binding of two SARS2 Positive control mAbs, our S2 subunit-specific RV82 and CR3022 antibody in ELISAs, see results. Two antibodies targeting NTD or RBD lost binding to this spike, compared with WT spike, as all mutations occur within NTD or RBD.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
Source:
HEK293 cells
Biosafety Level:
BSL-1
Organism:
SARS-Related Coronavirus 2 (virus caused COVID-19)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note: For research use only.
Reference: https://www.biorxiv.org/content/10.1101/2021.04.02.438218v1
