Human Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (spike RBD)
Catalog Number:
T11K48
Background:
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) caused the pandemic of COVID-19. The receptor-binding domain (RBD) subunit of the spike protein is to bind to its receptor on target cell surface (ACE2). A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 Spike RBD in human serum can facilitate research on antibodies produced in response to SARS-CoV-2 infection or vaccines.
Applications:
This kit is developed for serologic test for human IgG titer of Anti-SARS-CoV-2 Spike RBD subunit antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Description:
This kit was tested to bind to various published monoclonal antibodies, including CR3022 or CB6, starting from concentration of 10 μg/ml. COVID-19 convalescent sera were also tested starting from 100-fold dilution. See result. Please check ordering info for plate pre-coated with different spike RBD (WT or variants). Email us if you need a plate pre-coated with customized arrangement of spike RBD.
Ordering:
T11K48-WT for plate pre-coated with SARS-CoV-2 spike RBD of WT
T11K48-Beta for plate pre-coated with SARS-CoV-2 spike RBD of Beta strain
T11K48-Delta for plate pre-coated with SARS-CoV-2 spike RBD of Delta strain
T11K48-Omicron for plate pre-coated with SARS-CoV-2 spike RBD of Omicron strain
T11K48-BA.2 for plate pre-coated with SARS-CoV-2 spike RBD of Omicron BA.2 strain
T11K48-BA.2.12.1 for plate pre-coated with SARS-CoV-2 spike RBD of Omicron BA.2.12.1 strain
T11K48-BA.2.75 for plate pre-coated with SARS-CoV-2 spike RBD of Omicron BA.2.75 strain
T11K48-BA.4/5 for plate pre-coated with SARS-CoV-2 spike RBD of Omicron BA.4/5 strain
T11K48-XBB.1.5 for plate pre-coated with SARS-CoV-2 spike RBD of Omicron XBB.1.5 strain
Biosafety Level:
BSL-1
Organism:
SARS-Related Coronavirus 2 (virus caused COVID-19)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 Spike RBD human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with spike RBD protein, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
- Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
Recommendations:
Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Anti-SARS or SARS-CoV-2 S RBD Monoclonal Neutralizing Antibody (Cat. No. Z84N67).
