West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (NY99 strain)
Catalog Number:
A46U23
Description:
Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL 2 precautions, this WNV RVP can be handled using BSL-2 containment practices. Infection of cells with this RVP carrying Renilla luciferase reporter results in high level Renilla luciferase activity. See our titration result showing that the starting 2-fold diluted RVP-infected target cells (Vero) generates signal ~ 10,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of one known neutralizing monoclonal antibody– E60 that binds to domain DI/DII region of the viral envelope protein. See figure
Applications:
- Anti-West Nile Virus (WNV) neutralizing antibody screening at high throughput
- Anti-West Nile Virus (WNV) drug screening at high throughput
- West Nile Virus (WNV) vaccine efficacy evaluation at high throughput
- West Nile Virus (WNV) pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies, such as ADCC or ADE analysis
Biosafety Level:
Handle it in biosafety cabinet in BSL-2. Contacted tips & tubes should be decontaminated by 10% disinfecting bleach
Organism:
West Nile Virus (WNV)
Storage and shipping condition:
Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw.
Size:
200 μl with recommended 5-fold dilution. We recommend to use it at a dilution fold where the signal of RVP infection is 100-1,000-fold higher than uninfected control (cell alone as background). The vial is sufficient for 20 reactions (50 μl diluted virus).
Use protocol:
A complete protocol is supplied with the shipping of product.
Briefly, incubate 50 μl diluted virus with your sample in each well of 96-well plate with at least one duplicate for 30-60 min at 37°C. Then add 50 μl target cells (Vero or others expressing receptor). Read Renilla luciferase signal next day.
Note:
- Pseudoviruses are intended for Research Use Only
- A positive control mAb is included with pseudovirus purchase
