Swine Anti-Pseudorabies Virus Antibody IgG Titer Serologic Assay Kit (gB)

Swine Anti-Pseudorabies Virus Antibody IgG Titer Serologic Assay Kit (gB)

Catalog Number:

B29G36

Background:

Pseudorabies virus (PRV) belongs to the Herpesviridae family and is an important veterinary pathogen of domestic pigs. A rapid and effective assay kit detecting the levels of anti-PRV envelope protein in pig serum can facilitate research on antibodies produced in response to PRV infection or vaccines.

Applications:

This kit is developed for serologic test for swine IgG titer of anti-PRV envelope gB in serum/plasma or purified swine antibody (monoclonal & polyclonal) in vitro.

Description:

This kit was tested to bind to convalescent swine sera (n = 20) with sensitivity of 90%, specificity of 100% and Cohen’s kappa of 0.9 for PRV gB protein.

Biosafety Level:

BSL-1

Organism:

Pseudorabies virus (PRV)

Assay Principles:

This kit is developed for a competitive ELISAs (also known as inhibition or blocking) ELISA format, providing a rapid detection of anti-Pseudorabies virus (PRV) IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with indicated protein, Positive control, Negative Control, an HRP-Anti-PRV-gB secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
2. Dilute the Secondary antibody HRP-Anti-PRV-gB by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value inversely reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.