Human TIGIT Protein, Myc and Fc tag

 

Catalog Number:

U37J55

Description:

Human ITIM domains (TIGIT) is an immune receptor present on a fraction of T cells and natural killer (NK) cells. It binds to CD155 with higher affinity and CD112 with lower affinity. A recombinant form (Met 22 – Pro 141) of TIGIT protein (Accession: Q495A1) fused with Myc tag and human IgG1 Fc (Fc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined >95% as determined by reducing SDS-PAGE. We evaluated its binding to a TIGIT-specific antibody clone RV95 (Cat.No. S88S6) and Myc tag-specific antibody clone RV19 (Cat.No. M52T82) in ELISAs, see results.

Protein Synonyms:

TIGIT, VSIG9, VSTM3

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Met 22 – Pro 141

Tag:

Myc and Fc

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.