VSV Pseudotyped Rabies Virus G Fusion with Luciferase Reporter
Catalog Number:
U13T93
Description:
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full-length Rabies virus (RABV) glycoprotein (G) (NCBI: NP_056796.1). Rabies virus is a member of the family Rhabdoviridae and the genus Lyssavirus. The Rabies virus glycoprotein (RABV-G) is the only surface-exposed protein on the virus and enables the virus to bind to host cell receptors and fuse with the cell membrane to initiate infection, therefore RABV-G is the target of vaccine or neutralizing antibody. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal ~ 10,000-fold higher than uninfected control (cell alone as background in blue). Anti-Rabies Virus G Antibody (Domain III-specific) (clone RV30, Cat.No. N98S38) was tested to neutralize against this pseudovirus. See picture.
Applications:
- Anti-Rabies virus neutralizing antibody screening at high throughput
- Anti-Rabies virus drug screening at high throughput
- Rabies virus vaccine efficacy evaluation at high throughput
- Rabies virus pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies, such as ADCC analysis
Biosafety Level:
Handle it in biosafety cabinet in BSL-2. Contacted tips & tubes should be decontaminated by 10% disinfecting bleach
Organism:
Rabies virus
Storage and shipping condition:
Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw
Size:
200 μl with recommended 200-fold dilution. We recommend to use it at a dilution fold where the signal of RVP infection is 100-1,000-fold higher than uninfected control (cell alone as background). The vial is sufficient for 800 reactions (50 μl diluted virus).
Use protocol:
A complete protocol is supplied with the shipping of product.
Briefly, incubate 50 μl diluted virus with your sample in each well of 96-well plate for 60 min at 37°C. Then add 50 μl target cells, such as A549 or others expressing RABV receptor, followed by luciferase signal reading.
Note:
- Pseudoviruses are intended for Research Use Only
- A positive control mAb is included with pseudovirus purchase
