Human Recombinant Prostate-Specific Antigen (Kallikrein 3, Myc & His tags)
Catalog Number:
E37Z34
Description:
Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), P-30 antigen, is a glycoprotein enzyme encoded in humans by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland in men and the paraurethral glands. A recombinant form of PSA protein (sequence Ala18-Pro261) was produced by human embryonic kidney HEK cells, followed by purification. The molecular weight is 27.88 kDa without tags. This product contains a Myc tag followed by a 6 hexa-histidine tag at the C-terminus. The purity has been examined >90% as determined by reducing SDS-PAGE. We evaluated its binding to anti-His tag antibody (clone RV7, Cat.No. J96C86), anti-Myc tag antibody (clone RV19, Cat.No. M52T82), and a PSA-specific antibody (clone RV50, Cat.No. W24D36) in ELISAs, see results.
Protein Synonyms:
Prostate-Specific Antigen, PSA, Semenogelase, APS, Seminin, Gamma-Seminoprotein, P-30 Antigen, Kallikrein-3, KLK3
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays
Source:
Human embryonic kidney HEK cells
Species:
Human
Sequence:
Ala18-Pro261
Tag:
Myc & His
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
