Mouse Anti-Japanese Encephalitis Virus (JEV) Envelope Antibody IgG Titer Serologic Assay Kit
Catalog Number:
V57L26
Background:
Japanese encephalitis virus (JEV) is a mosquito-borne virus from the genus Flavivirus in the family Flaviviridae. The structural Envelope (E) (JEV-E) is the major target of vaccine or neutralizing antibody development. A rapid and effective assay kit detecting the levels of anti-JEV-E in mouse serum can facilitate research on antibodies produced in response to JEV infection or vaccines.
Applications:
This kit is developed for serologic test for mouse IgG titer of Anti-JEV-E-specific antibody in serum/plasma or purified mouse antibody (monoclonal & polyclonal) in vitro. It is for research use only.
Description:
These kits were tested to measure monoclonal antibodies – 4G2 (Cat. No. E40Q92) or E60, see figure.
Biosafety Level:
BSL-1
Organism:
Japanese encephalitis virus (JEV)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-JEV-E mouse IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with JEV-E (from Peking 3 strain), Positive control, Negative Control, an HRP-Anti-Mouse IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
- Dilute the Secondary antibody HRP-Anti-Mouse IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
Recommendations:
Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Mouse Anti-Flavivirus Envelope Protein Antibody 4G2 (Cat. No. E40Q92)
