Anti-WNV-E Human Monoclonal Antibody (E16 similar)
Catalog Number:
V53R88
Clone:
RV86
Description:
Clone E16 mouse antibody was known to react with the domain III lateral ridge (DIII-LR) of the envelope (E) protein of West Nile Virus (WNV), and show neutralizing against multiple strains of WNV in vitro. Here we provide E16 similar human antibody that has been tested to strongly bind to WNV-E (Cat. No. R36G82), but not JEV-E (Cat. No. K63C58), DENV1-E (Cat. No. P76A64-S1), DENV2-E (Cat. No. P76A64-S2), ZIKV-E (Cat. No. G69R89), see figure. It has also been tested to neutralize WNV Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (NY99 strain) (Cat. No. A46U23), see figure.
Epitope:
The domain III lateral ridge (DIII-LR) of WNV-E
Clonality and isotype:
Monoclonal antibody human IgG1
Specificity:
West Nile Virus (WNV)
Form:
Liquid in PBS, pH7.2, without preservatives
Storage:
Shipped at ambient temperature. Stored at 4 °C.
Purification:
Cell culture supernatant followed by filtration and Protein A purification
Applications:
IF, WB, ELISA, IHC, IP, Neutralization control et al. For Western blotting, the suggested use of this reagent is 0.1 – 0.5 μg/ml. For ELISA, the suggested use is 1-10 μg/ml. For immunocytochemistry, a concentration range of 0.1 – 0.5 μg/ml is recommended. For neutralization assay, the suggested concentration is 0.1-50 μg/ml. It is recommended that the reagent should be titrated for optimal performance by the user.
Conjugate:
Unconjugated
Product Usage Statement:
This product is intended for research use only.
