Human Anti-MERS Antibody IgG Titer Serologic Assay Kit (Trimeric spike)

Human Anti-MERS Antibody IgG Titer Serologic Assay Kit (Trimeric spike)

Catalog Number:

C7C45

Background:

Middle East Respiratory Syndrome (MERS) caused-infection was first reported in Saudi Arabia in 2012 and later occurred in South Korea in 2015. A rapid and effective assay kit detecting the levels of anti-MERS Spike in human serum can facilitate research on antibodies produced in response to MERS infection or vaccines.

Applications:

This kit is developed for serologic test for human IgG titer of Anti-MERS Spike antibody in serum/plasma or purified human antibody (monoclonal & polyclonal) in vitro. It is for research use only.

Description:

This kit was tested to bind to published monoclonal antibody (MERS27) starting from concentration of 10 μg/ml.

Biosafety Level:

BSL-1

Organism:

Middle East Respiratory Syndrome (MERS)

Assay Principles:

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-MERS Spike human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with trimeric spike protein, Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps):

1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
2. Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.

Recommendations:

Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Anti-MERS S NTD Monoclonal Neutralizing Antibody (Cat. No. B73K78).