MERS-CoV Trimeric Spike protein (His and Twin-Strep tags)
Catalog Number:
U97O74
Description:
A recombinant trimeric form of the spike glycoprotein (S protein) of MERS-Cov (GenBank: JX869059.2) was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (V1060P and L1061P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition and Native blue gels, with results proving trimerization and glycosylation. We also evaluated the binding of MERS-specific mAb (clone RV45, Cat. No. B73K78) and published MERS27 antibody in ELISAs, see results.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
Source:
HEK293
Biosafety Level:
BSL-1
Organism:
MERS-Related Coronavirus (virus caused 2015 outbreak in Republic of Korea)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
