Swine Anti-African Swine Fever Virus Envelope Antibody IgG Titer Serologic Assay Kit
Catalog Number:
J8G58
Background:
African swine fever (ASF) caused by the African swine fever virus (ASFV) infection is a highly contagious and lethal disease of domestic pigs. ASFV is the only member of the Asfarviridae. A rapid and effective assay kit detecting the levels of anti-ASFV envelope protein in pig serum can facilitate research on antibodies produced in response to ASFV infection or vaccines.
Applications:
This kit is developed for serologic test for swine IgG titer of anti-ASFV envelope protein in serum/plasma or purified swine antibody (monoclonal & polyclonal) in vitro.
Description:
This kit was tested to bind to convalescent swine sera (n = 50) with sensitivity of 100%, specificity of 80% and Cohen’s kappa of 0.6.
Biosafety Level:
BSL-1
Organism:
African Swine Fever Virus (ASFV)
Assay Principles:
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti- African Swine Fever Virus (ASFV) IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with indicated protein, Positive control, Negative Control, an HRP-Anti-Swine IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
- Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37°C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml)
- Dilute the Secondary antibody HRP-Anti-Swine IgG by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5-1 hour. Avoid light by foil. Wash wells as mentioned.
- Add TMB to each well (0.1 ml) and incubate at room temperature for 5-30 minutes.
- Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
Storage and shipping condition:
Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.
