Quick Human HER2 /ErbB2 ELISA Kit

Catalog Number:

K43J75

Background:

Human epidermal growth factor receptor 2 (HER2 /ErbB2) is an important biomarker and tumor marker used for targeted treatments of specific types of cancer.

Applications:

This kit is developed to quickly titrate human HER2/ErbB2 sample in high throughput. It is for research use only.

Description:

These kits were validated using human HER2 protein (see figure). The detection linear range is 0.5 – 294 ng/ml; Sensitivity is 0.5 ng/ml.

Biosafety Level:

BSL-1

Organism:

Human epidermal growth factor receptor 2 (HER2/ErbB2)

Assay Principles (Take neutralizing antibody as example):

This kit is developed for a sandwich ELISA format to specifically capture human HER2/ErbB2 protein in serum, plasma, tissue homogenates, cell culture supernatant and other biological fluids. The whole process should take about 2.5 hours. An antibody specific for HER2/ErbB2 has been pre-coated onto a detachable 96-well microtiter plate. The standards and test samples are added into the wells and the HER2/ErbB2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with HRP labeled detector antibody, which binds the captured HER2/ErbB2 present in each well. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-colored product that changes to yellow after addition of stop solution. The density of yellow is proportional to the amount of ErbB2 / HER2 captured in each well. The concentration of HER2/ErbB2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

The kit consists of detachable 96-well plate pre-coated with HER2/ErbB2-specific antibody, HER2/ErbB2 protein standard, HRP labeled detector antibody, TMB and sample dilution/wash/stop buffer.

Brief protocol (detailed protocol is provided with the product):

1. Add your samples containing human HER2/ErbB2 and provided purified HER2/ErbB2 protein standard into pre-coated plate (or strips based on sample size). Incubate at 37°C for 1 hour.
2. Wash wells. Add HRP labeled detector antibody to wells. Incubate at room temperature for 1 hour.
3. Wash wells. Add TMB and stop solution. Read absorbance (OD) at 450 nm. The OD Value reflects the amount of HER2/ErbB2 in the samples, which is then quantified against HER2/ErbB2 standard curve to calculate titer.

Storage and shipping condition:

Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2°C to 8°C. Avoid light. The shelf life is 60 days from the date of opening.