Human Cytomegalovirus (HCMV) Trimeric Glycoprotein B (gB) protein

Catalog Number:

K82M85

Description:

Human Cytomegalovirus (HCMV) is a highly complex virus harboring more than 20 different glycoproteins in its envelope. The glycoprotein B is the major envelope protein inducing neutralizing antibody response. A recombinant HCMV glycoprotein B (gB) (GenBank: ACZ79977.1) consists of ectodomain (Ala 21-Gln 690) and was produced by insect cells, followed by purification. This product contains N-terminal Myc tag and C-terminal trimerization motif, human Fc tag followed by a 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition, where the target protein with a calculated MW of ~82 kDa (including tags) was shown. We evaluated its binding to a HCMV gB-specific antibody (clone RV22, Cat. No. D53Y20) and His tag-specific antibody (Cat.No. J96C86) as well as Myc tag-specific antibody (Cat.No. M52T82) in ELISAs, see results.

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others

Source:

Insect cells

Biosafety Level:

BSL-1

Organism:

Human Cytomegalovirus (HCMV)

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.