Ebola SUDV Trimeric GP protein –TwinStrep and His tag
Catalog Number:
B78A28
Description:
A recombinant Ebola SUDV GPΔmuc protein (derived from strain Boniface) consists of resides 33-313 and 473-637 (MLD residues 314-472 and transmembrane domain residues 638-676 deleted) and was produced by insect cells, followed by purification. This product contains C-terminal trimerization motif, enterokinase cleavage, a TwinStrep tag and 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition and native blue gel. We evaluated the binding of HIV negative control mAb and one published antibody CA45 (Zhao, et al, Cell 2017, 169) in ELISAs, see results.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others
Source:
Insect cells
Biosafety Level:
BSL-1
Organism:
Filovirus Ebola Sudan-Boniface strain
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
