Ebola Zaire Makona Trimeric GP protein –TwinStrep and His tags

Catalog Number:

P54P89

Description:

A recombinant Ebola Zaire GPΔmuc protein (derived from strain Makona) consists of resides 32-309 and 461-628 (MLD residues 310-460 and transmembrane domain downstream of residue 628 deleted) and was produced by insect cells, followed by purification. This product contains C-terminal trimerization motif, enterokinase cleavage (DDDDK), a TwinStrep tag and 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition. We evaluated the binding of three published antibodies-KZ52, FVM04 and CA45 (Zhao, et al, Cell 2017, 169) in ELISAs, see results.

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others

Source:

Insect cells

Biosafety Level:

BSL-1

Organism:

Filovirus Ebola Zaire-Makona strain

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.