Lenti-Pseudovirus_SARS-CoV Spike with luciferase reporter
Catalog Number:
U85H22
Description:
This pseudotyped virus uses lentiviral vectors to package S protein of SARS-Cov (GenBank: DQ412594.1), which was designed by codon optimizing the full-length S sequence for mammalian expression. Infection of cells with this pseudotyped virus carrying luciferase reporter results in detectable luciferase activity. See our titration result showing the undiluted pseudovirus (green) generates signal 10,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of SARS Positive control mAb and 2 published neutralizing mAbs (CR3022 and 80R) by using this pseudotyped virus, see results.
Applications:
- Anti-SARS-CoV neutralizing antibody screening at high throughput
- Anti-SARS-CoV drug screening at high throughput
- SARS vaccine efficacy evaluation at high throughput
- SARS-CoV pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies, such as ADCC analysis
Biosafety Level:
Handle it in biosafety cabinet in BSL-2. Contacted tips & tubes should be decontaminated by 10% disinfecting bleach
Organism:
SARS-Related Coronavirus (virus caused 2003 outbreak)
Storage and shipping condition:
Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend no freeze after thaw
Size:
- ml with recommended 30-fold We recommend to use it at a dilution fold where the signal of pseudovirus infection is 100-fold higher than uninfected control (cell alone as background). Can be used for 825 reactions with reduced amount of pseudovirus (40 μl diluted virus).
Use protocol:
The complete protocol is shipped with the product.
Briefly, incubate 40 μl diluted virus with 10 μl your sample in each well of 96-well plate for 30-60 min at 37°C. Then add 25 μl target cells (such as 293T-ACE2 stable cell line). Add 150ul fresh media next day and read signal on Day 3.
Note:
Pseudoviruses are intended for Research Use Only
