HIV-1 Trimeric GP140 protein-clade B, YU2 strain
Catalog Number:
W44H24
Description:
A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from YU2 strain (clade B, GenBank: QHJ92204) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein with furin cleavage site knocked-down, T4 foldon trimerization domain, and C-terminal 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been evaluated by Native Blue gel and Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE for purity as well as ELISAs for binding, see results. A rabbit antibody 11B that targets 241 glycan hole (BG505 specific, catalog U90R79) does not to bind to this protein (ref: Cell Rep. 2016;16(9):2327-38.).
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
Source:
HEK293 cells
Biosafety Level:
BSL-1
Organism:
HIV-1 (virus caused AIDS)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
