VSV-Pseudovirus_Bourbon Virus GP with Luciferase Reporter

Catalog Number:

J19C46

Description:

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Bourbon virus (BRBV) (GenBank: AMN92169.1). As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal ~ 100,000-fold higher than uninfected control (cell alone as background in blue), see picture.

Applications:

• Anti-Bourbon virus neutralizing antibody screening at high throughput
• Anti-Bourbon virus drug screening at high throughput
• Bourbon virus vaccine efficacy evaluation at high throughput
• Bourbon virus pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies, such as ADCC analysis

Biosafety Level:

Handle it in biosafety cabinet in BSL-2. Contacted tips & tubes should be decontaminated by 10% disinfecting bleach

Organism:

Orthomyxovirus, genus of Thogotovirus-Bourbon virus (BRBV)

Storage and shipping condition:

Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw

Size:

200 μl with recommended ~50-fold dilution. We recommend using it at a dilution fold where the signal of pseudovirus infection is 1,000-fold higher than uninfected control (cell alone as background), although 100-1,000-fold high is acceptable. Can be used for 100 reactions (100 μl diluted virus) or 250 reactions if using reduced amount of pseudovirus (40 μl diluted virus).

Use protocol:

The complete protocol is shipped with the product.

Briefly, incubate 100 μl diluted virus with 100 μl your sample in each well of 96-well plate for 30-60 min at 37°C. Then add 100 μl target cells (Vero-CCL81 or others with receptor). Read luciferase signal next day. For protocol using reduced amount, incubate 40 μl diluted virus with 10 μl your sample in each well of 96-well plate for 30-60 min at 37°C. Then add 25 μl target cells. Add 150ul fresh media next day and read signal on Day 3.

Note:

Pseudoviruses are intended for Research Use Only