VSV-Pseudovirus_Chandipura Virus G with Luciferase Reporter

Catalog Number:

P79K59

Description:

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry Glycoprotein (G) of Chandipura virus (CHPV) (GenBank: YBP03243.1). As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal ~ 1,000,000-fold (A549 cell line) higher than uninfected control (cell alone as background in blue), see picture.

Applications:

• Anti-Chandipura virus neutralizing antibody screening at high throughput
• Anti-Chandipura virus drug screening at high throughput
• Chandipura virus vaccine efficacy evaluation at high throughput
• Chandipura virus pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies, such as ADCC analysis

Biosafety Level:

Handle it in biosafety cabinet in BSL-2. Contacted tips & tubes should be decontaminated by 10% disinfecting bleach

Organism:

Rhabdovirus- Chandipura virus (CHPV)

Storage and shipping condition:

Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw

Size:

200 μl with recommended ~300-fold dilution. We recommend using it at a dilution fold where the signal of pseudovirus infection is 1,000-fold higher than uninfected control (cell alone as background), although 100-1,000-fold high is acceptable. Can be used for 600 reactions (100 μl diluted virus) or 1,500 reactions if using reduced amount of pseudovirus (40 μl diluted virus).

Use protocol:

The complete protocol is shipped with the product.

Briefly, incubate 100 μl diluted virus with 100 μl your sample in each well of 96-well plate for 30-60 min at 37°C. Then add 100 μl target cells (A549, Vero E6 or others with receptor). Read luciferase signal next day. For protocol using reduced amount, incubate 40 μl diluted virus with 10 μl your sample in each well of 96-well plate for 30-60 min at 37°C. Then add 25 μl target cells. Add 150ul fresh media next day and read signal on Day 3.

Note:

Pseudoviruses are intended for Research Use Only