Japanese Encephalitis Virus Envelope Glycoprotein (E protein) (Peking 3 Strain), His and Fc tags 

Catalog Number:

X16Y90

Description:

Japanese encephalitis virus (JEV) is a flavivirus and spread primarily by mosquitoes. A recombinant JEV envelope glycoprotein (E protein) (Peking 3 Strain, GenBank: AAB18951.1) consists of ectodomain and was produced by insect cells, followed by purification. This product contains C-terminal human Fc tag and a 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition, where the target protein with a calculated MW of ~75 kDa (including tags) was shown. We evaluated its binding to His tag-specific antibody (Cat.No. J96C86), pan-flavivirus antibody 4G2 (Cat.No. E40Q92) and cross-reactive antibody E60, in ELISAs, see results.

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others

Source:

Insect cells

Biosafety Level:

BSL-1

Organism:

Japanese encephalitis virus (JEV)

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.