Single or Bulk Cell Sorting Using FACS

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Fluorescence-activated cell sorting is a type of flow cytometry for sorting a group of cells with specific light scattering and fluorescent characteristics. The cell sample is stained with fluorescence antibodies for cell surface markers and entrained into a nozzle to form droplets, followed by laser-based fluorescence analysis. The fluorescent character of interest of each cell is electrical charged and sorted by the opposite charge on the deflection plates. The sorted cells are phenotype defined with great homology. The sorted cell can be collected in single or bulk format.