Human CD8 Alpha&Beta (CD8A&CD8B) Heterodimer Protein
Catalog Number:
W30Y61
Description:
Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein was produced by human embryonic kidney HEK cells, followed by purification. CD8 alpha (CD8A ectodomain, Ser 22 – Asp 182, Accession: P01732-1) was fused with a domain called “key domain” (proprietary technology), followed with HRV3C cleavage and Myc and Twin-Strep tags at the C-terminus. CD8 beta (CD8B ectodomain, Leu 22 – Pro 170, Accession: P10966-1) was fused with a domain called “lock domain” (proprietary technology), followed with HRV3C cleavage and 6 hexa-histidine tag at the C-terminus. The binding between key domain and lock domain generates heterodimer of CD8 alpha&beta. The purity has been examined by reducing SDS-PAGE, where protein migrates ~62 kDa. We evaluated its binding to anti-His tag antibody (clone RV7, Cat.No. J96C86), anti-Myc tag antibody (clone RV19, Cat.No. M52T82), and a human CD8A-specific antibody (clone RV65, Cat.No. U18G96) in ELISAs. This protein simultaneously binding to His and Myc tag-specific antibodies validates the heterodimeric conformation. The protein can be cleaved using HRV3C for tag-free.
Protein Synonyms:
CD8 alpha & beta, CD8 α&β, CD8A & CD8B
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays
Source:
Human embryonic kidney HEK cells
Species:
Human
Sequence:
CD8 alpha (Ser 22 – Asp 182) and CD8 beta (Leu 22 – Pro 170)
Tag:
His, Myc and Strep
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
