Monkeypox Virus Dimeric A35R protein (2022 Strain, Myc and His tags)

Price range: $288.00 through $969.00

A recombinant form of monkeypox virus protein A35R (Genbank: ON563414.3, MPXV_USA_2022_MA001; ON838178.1, isolate SI2022_S7) was produced by insect cells, followed by purification.

Monkeypox Virus Dimeric A35R protein (2022 Strain, Myc and His tags)

Catalog Number:

S95L46

Description:

Monkeypox virus (MPXV) is a member of the Orthopoxvirus of the family Poxviridae. A35R extracellular enveloped virus (EEV) envelope glycoprotein is needed for formation of actin-containing microvilli and cell-to-cell spread of virion EEV membrane phosphoglycoprotein, similar to Vaccinia virus strain Copenhagen A33R. A33 contains a membrane-proximal cysteine on ectodomain that forms an intermolecular disulfide bridge.

A recombinant form of monkeypox virus protein A35R (Genbank: ON563414.3, MPXV_USA_2022_MA001; ON838178.1, isolate SI2022_S7) was produced by insect cells, followed by purification. This product contains ectodomain only (58-end), N-terminal Myc tag and C-terminal 6 hexa-histidine tag with amino acid sequence available upon request.  The ectodomain includes cysteines for dimer formation. Amino acid sequence for A35R: lnqcmsankaaitdsavavaaassthrkvvssttqydhkescnglyyqgscyilhsdyksfedakancaaesstlpnksdvlttwlidyvedtwgsdgnpitkttsdyqdsdvsqevrkyfct. The monomeric form of this product is predicted to be a molecular mass of ~17 kDa.

This product has been run on SDS-PAGE with reduced condition. We also evaluated its binding to antibodies of anti-His, anti-Myc or clone RV10. The dimeric conformation was evaluated with SEC (Superose 6 column) with two peaks that we assigned one as a dimer fraction peak. The product is not a SEC fraction.

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others

Source:

Insect cells

Biosafety Level:

BSL-1

Organism:

Monkeypox virus 2022 Strain

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.

Weight 7 lbs
Dimensions 11 × 11 × 11 in
Size

100 μg, 500 μg

monkeypox virus dimeric a35r protein (2022 strain, myc and his tags)Monkeypox Virus Dimeric A35R protein (2022 Strain, Myc and His tags)
Price range: $288.00 through $969.00Select options
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