SARS-CoV-2 Delta Trimeric Spike protein (His and Twin-Strep tags)

Price range: $491.00 through $1,299.00

A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (GenBank: MN908947) bearing mutations identified in B.1.617.2 (Delta strain) was produced by human embryonic kidney HEK293 cells, followed by purification.

SARS-CoV-2 Delta Trimeric Spike protein (His and Twin-Strep tags)

Catalog Number:

G30X55

Description:

A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-CoV-2 (GenBank: MN908947) bearing mutations of T19R, K77R, G142D, Δ156-157, R158G, A222V, L452R, T478K, D614G, P681R, and D950N, identified in variant of B.1.617.2 (Delta strain), was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition with result showing pure and expected size. We also evaluated the binding of two Positive control mAbs in ELISA, see results.

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe

Source:

HEK293 cells

Biosafety Level:

BSL-1

Organism:

SARS-Related Coronavirus 2 (virus caused COVID-19)

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.

Weight 7 lbs
Dimensions 11 × 11 × 11 in
Size

100 μg, 500 μg

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sars cov 2 delta trimeric spike protein (his and twin strep tags)SARS-CoV-2 Delta Trimeric Spike protein (His and Twin-Strep tags)
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