EpiCD™ CHO Expression
Catalog Number:
M13U59
Description:
EpiCD™ CHO expression medium is a chemically-defined (CD), free of serum, proteins or growth factors, animal-origin components, and hydrolysates sterile, ready-to-use medium for suspension culture of Chinese hamster ovary (CHO) cell lines to achieve rapid cell growth, high density culture and efficient transient transfection.
EpiCD™ CHO Feed (Cat. No.M29V67) is designed for use with this medium to extend cell survival time and improve protein quality and metabolite profile, particularly post transfection.
Applications:
This medium supports multiple applications including:
- Support CHO suspension culture: CHO-K1, CHO-DG44, CHO-S and CHO-K1SV.
- Compatible with a variety of commonly used transfection reagents, such as PEI, Lipofectamine, 293fectin, TransIT-PRO with boost.
- Scalable production of recombinant protein or antibody to gram-scale yields.
Specifications:
| Appearance (liquid) | Orange yellow clear liquid |
| pH | 6.9-7.6 |
| Osmolality（mOsm/kg） | 270-330 |
| Storage | 2 – 8℃, dark and dry |
Culture Conditions:
- Cell medium: EpiCD™ CHO Expression medium, sterile liquid, doesn’t contain HT or L-glutamine. L-glutamine and Anti-Clumping Agent can be added per user.
- Cell line: CHO based cell lines for suspension cultures.
- Culture type: Suspension culture.
- Culture container: shake flasks, TPPs, bioreactors, etc.
- Temperature range: 37℃±5, temperature shift as needed for cell culture conditions.
- Shaker incubator setting: 5 – 8% CO2, ≥ 80% humidity, shaker speed 110-150rpm (amplitude: 50mm)
Ordering:
- M13U59-1L for 1 liter liquid.
- M13U59-10L for 10 liter powder. Easy reconstitution without additives required. See below steps.
- Larger pack sizes available upon request.
Powder Reconstitution (briefly)
- Use 24 g/L of ultrapure water with low ion concentration or WFI.
- Mix for a minimum of 40 min by stirring.
- Pass 0.22µm filter. Ready to use.
Product Statement:
For Further Manufacturing or Research Use. Not For Diagnostic or Therapeutic Use.
Legal Information:
EpiCD is a trademark of ReVacc Inc, USA
Free Sample:
Free Sample is available per request, fill this form
