EpiCD™ HEK293
Catalog Number:
N40H22
Description:
EpiCD™ HEK293 medium is a chemically-defined (CD), free of serum, proteins or growth factors, animal-origin components, and hydrolysates sterile, ready-to-use medium for suspension culture of HEK293 cell lines to achieve rapid cell growth, high density culture and efficient transfection.
Applications:
This medium supports multiple applications including:
- Support HEK 293 suspension culture: >4 x 106 cells/mL for FreeStyle 293-F or >14 x 106 cells/mL for Expi293 at viability >90%.
- Compatible with a variety of commonly used transfection reagents, such as PEI, Lipofectamine, 293fectin, TransIT-PRO with boost. See picture for examples of products we manufactured using this medium.
- Scalable production of recombinant protein or antibody to gram-scale yields.
- Scalable production of viral vectors, such as adeno-associated viruses (AAV), lentiviruses (LVV).
Specifications:
| Appearance (liquid) | Orange red clear liquid |
| pH | 6.9-7.6 |
| Osmolality（mOsm/kg） | 270-330 |
| Storage | 2 – 8℃, dark and dry |
| Shelf Life | 1 year from date of manufacture |
Culture Conditions:
- Cell medium: EpiCD™ HEK293, sterile liquid, ready to use (contains 4mM of L-glutamine)
- Cell line: HEK293 based cell lines for suspension cultures.
- Culture type: Suspension culture.
- Culture container: shake flasks, TPPs, bioreactors, etc.
- Temperature range: 37℃±5, temperature shift as needed for cell culture conditions.
- Shaker incubator setting: 5 – 8% CO2, ≥ 80% humidity, shaker speed 110-150rpm.
Ordering:
- N40H22-1L for 1 liter liquid.
- N40H22-10L for 10 liter powder. Easy reconstitution without additives required. See below steps.
- Larger pack sizes available upon request.
Powder Reconstitution (briefly)
- Use 24 g/L of ultrapure water with low ion concentration or WFI.
- Mix for a minimum of 40 min by stirring.
- Pass 0.22µm filter. Ready to use.
Product Statement:
For Further Manufacturing or Research Use. Not For Diagnostic or Therapeutic Use.
Legal Information:
EpiCD is a trademark of ReVacc Inc, USA
Free Sample:
Free Sample is available per request, fill this form
