AAV-CMV-Luciferase

Catalog Number:

K55V14

Description:

Adeno-associated virus (AAV) is a single stranded DNA virus and belongs to Parvoviridae family. AAV is packaged in a non-enveloped icosahedral capsid consisting of VP1, VP2 and VP3 proteins. AAV with features of non-pathogenic and no integrating into the host genome can be used to express genes of interest into cells or organism. Tissue specificity is determined by the capsid serotype and pseudotyping of AAV vectors (or called recombinant AAVs, rAAVs) to alter their tropism range is important to guide AAV to deliver gene of interest to the target organism. AAV related products have been extensively evaluated in human with multiple approved by the FDA, therefore, they are safe.

This panel of products uses CMV promoter and contains various serotype of AAV capsid (see catalog) to deliver fire luciferase gene. The titer was measured using qPCR (The titer may vary from lot) and verified using Firefly Luciferase Reporter Assay System (Cat. No. S78P56), see figure for AAV2-CMV-Luciferase.

Other AAV products with different promoter are available per request for AAV packaging service. Contact us!

Applications:

• Anti-AAV neutralizing antibody screening at high throughput
• Serve controls for AAV in-vivo study, CRISPR genome editing, optogenetics, chemogenetics and others
• AAV pseudovirus transduction of target cells for viral entry, receptor recognition, cellular tropism and functional studies
• Others

Organism:

Recombinant AAV carrying fire luciferase gene with CMV promoter

Purification:

Iodixanol gradient ultracentrifugation

Size and buffer:

50 μl in buffer of PBS + 0.001% Poloxamer 188

Storage and shipping condition:

Shipping with dry ice. Require -80°C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw

Order (varied by AAV capsid):

Cat. No.               Product name                                                      

K55V14-1             AAV1-CMV- Luciferase

K55V14-2             AAV2-CMV- Luciferase

K55V14-5             AAV5-CMV- Luciferase

K55V14-6             AAV6-CMV- Luciferase

K55V14-8             AAV8-CMV- Luciferase

K55V14-9             AAV9-CMV- Luciferase

K55V14-R            AAV2 retro-CMV-Luciferase

Note:

This product is intended for Research Use Only