Human GPC3 Protein, Myc and Fc tag

 

Catalog Number:

K27X31

Description:

Human Glypican-3 (GPC3) is a membrane-bound protein highly expressed in hepatocellular carcinoma (HCC) but absent in normal adult liver tissue, making it a key biomarker and therapeutic target. A recombinant form (Gln 25 – His 559) of GPC3 protein (Accession: P51654) fused with Myc tag and human IgG1 Fc (Fc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined as determined by reducing SDS-PAGE, where protein with a calculated MW of ~ 92 kDa (including tags) was observed. We evaluated its binding to Myc tag-specific antibody clone RV19 (Cat.No. M52T82) in ELISAs, see results.

Protein Synonyms:

GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS, SGB, SGBS, SGBS1

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Gln 25 – His 559

Tag:

Myc and Fc

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.