Human ErbB3 / HER3 Protein-Myc/His tag or tag free
Catalog Number:
R44A32
Description:
Human ErbB3 / HER3 Protein was produced by human embryonic kidney HEK cells, followed by purification. This recombinant ErbB3 / HER3 (Ser 20 – Thr 643, Accession: P21860-1) was fused with HRV3C cleavage and followed with Myc and 6 hexa-histidine tag at the C-terminus. This design provides an option for the protein to be cleaved using HRV3C for tag-free. The purity has been examined by reducing SDS-PAGE, where protein migrates ~70 kDa. We evaluated its binding to anti-His tag antibody (clone RV7, Cat.No. J96C86), anti-Myc tag antibody (clone RV19, Cat.No. M52T82), and a human HER3-specific antibody (clone RV68, Cat.No. V94U51) in ELISAs, see figure.
Protein Synonyms:
ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays
Source:
Human embryonic kidney HEK cells
Species:
Human
Sequence:
ErbB3 / HER3 (Ser 20 – Thr 643)
Tag:
Myc and His tags with HRV3C cleavage site for tag free
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
