Dengue Virus 2 E Protein – Myc and His tags
Catalog Number:
W80T78
Description:
A recombinant form of envelope (E) protein from Dengue virus serotype 2 (DENV2) (strain 16681, GenBank: AAA73185) was produced by insect cells, followed by purification. This product contains N-terminal Myc tag and C-terminal 6 hexa-histidine tag. Amino acid sequence is available upon request. This product has been examined on SDS-PAGE gel under reduced condition with > 95% purity (estimated MW 46 kD). We evaluated its binding to anti-His tag antibody clone RV7 (Cat. No. J96C86) or Anti-Myc Tag Antibody clone RV19 (Cat. No. M52T82) or Anti-Dengue E Domain III Neutralizing Antibody Clone RV25 (Cat. No. K34Q76) in ELISAs, see results.
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and others
Source:
Insect cells
Biosafety Level:
BSL-1
Organism:
Dengue virus (serotype 2)
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
