Human PD-L1 Protein, Fc Tag
Catalog Number:
Z25Y86
Description:
Human PD-L1 (programmed death ligand 1) is commonly expressed on dendritic cells and macrophages, and its ectodomain is composed of a membrane distal IgSF V-set and a membrane proximal IgSF C-set domain. PD-L1 is often overexpressed in different tumors, and its interaction with PD-1 on T cells enables cancer cells to evade T-cell-mediated immune responses. A recombinant form (Phe 19 – Arg 238) of PD-L1 protein (Accession: NP_054862.1) fused with human IgG1 Fc (Fc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. Sequence is available upon the request. The purity has been examined >95% as determined by reducing SDS-PAGE, where protein migrates as 60-75 kDa. We evaluated its binding to a PD-L1-specific antibody (clone RV59, Cat.No. F16S89) in ELISAs, see results.
Protein Synonyms:
Human PD-L1 (programmed death ligand 1), B7-H1, CD274, PDCD1L1, PDCD1LG1
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays
Source:
Human embryonic kidney HEK cells
Species:
Human
Sequence:
Phe 19 – Arg 238
Tag:
human IgG1 Fc tag
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
