Human VEGFR2 Protein, His tag
Catalog Number:
J82W46
Description:
Vascular endothelial growth factor (VEGF) is one of the key players in angiogenesis, the process of new blood vessel formation. VEGF plays role through its two receptors, VEGFR1 and VEGFR2, expressed in endothelial cells. Blocking the interaction between VEGF and its receptors using specific antibodies or other protein entities to prevent signaling has been one of many approaches to reduce tumor growth. A recombinant form (Ala 20 – Glu 764) of VEGFR2 protein (Accession: P35968) was produced by human embryonic kidney HEK cells, followed by purification. This product contains a 6 hexa-histidine tag at the C-terminus. The purity has been examined >90% as determined by reducing SDS-PAGE, where protein migrates as 98 kDa. We evaluated its binding to anti-His tag antibody (clone RV7, Cat.No. J96C86), and a VEGFR2-specific antibody (clone RV62, Cat.No. P11C36) in ELISAs, see results.
Protein Synonyms:
CD309, Flk-1, Flk1, Kdr, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase NYK, Protein-tyrosine kinase receptor flk-1, FLK-1
Applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays
Source:
Human embryonic kidney HEK cells
Species:
Human
Sequence:
Ala 20 – Glu 764
Tag:
His
Size and Formulation:
100 or 500 μg in PBS, pH7.2, without preservatives.
Note: Not lyophilized formulation, so no lyophilized protectant reagents added.
Endotoxin:
Less than 1.0 EU per μg by the LAL method / rFC method.
Shipping or storage condition
Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term. Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.
Note:
For research use only.
